PotentinhibitorofTGF-βtypeIreceptorALK5kinase,typeIactivin/nodalreceptorALK4andtypeInodalreceptorALK7(IC50valuesare12,45and7.5nMrespectively).BlocksphosphorylationofSmad2andinhibitsTGF-β-inducedepithelial-to-mesenchymaltransition.OnlyweaklyinhibitsALK-1,-2,-3,-6andMAPKactivity.MorepotentthanSB431542(Cat.No.1614).Inhibitsdifferentiationofratinducedpluripotentstemcells(riPSCs)andincreasesclonalexpansionefficiency.Helpsmaintainhomogeneityandlong-terminvitroself-renewalofhumaniPSCs.
A83-01isalsoofferedaspartoftheTocriscreenStemCellToolbox.FindoutmoreaboutcompoundlibrariesavailablefromTocris.
M.Wt | 421.52 |
Formula | C25H19N5S |
Storage | Storeat-20°C |
Purity | ≥98%(HPLC) |
CASNumber | 909910-43-6 |
PubChemID | 16218924 |
InChIKey | HIJMSZGHKQPPJS-UHFFFAOYSA-N |
Smiles | S=C(NC5=CC=CC=C5)N(N=C3C4=NC(C)=CC=C4)C=C3C2=CC=NC1=CC=CC=C12 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solvent | MaxConc.mg/mL | MaxConc.mM | |
---|---|---|---|
Solubility | |||
DMSO | 21.08 | 50 |
Thefollowingdataisbasedontheproductmolecularweight421.52.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
---|---|---|---|
1mM | 2.37mL | 11.86mL | 23.72mL |
5mM | 0.47mL | 2.37mL | 4.74mL |
10mM | 0.24mL | 1.19mL | 2.37mL |
50mM | 0.05mL | 0.24mL | 0.47mL |