Fluorescentdye;suppliedasanNHSesterforcouplingtoprimaryaminegroups.CompatIBLewithself-labelingtagsystems(e.g.HaloTag®andSNAPtag®).Suitableforconfocalfluorescentimaging,superresolutionmicroscopy(SRM)techniquessuchasdSTORM(liveandfixedcells)andPAINTimaging.CanbemultiplexedfortwocolourimagingwithJaneliaFluor®549SE(Cat.No.6147).Cellpermeable.Excitationmaximum=646nm;emissionmaximum=664nm.Quantumyield=0.54,Extinctioncoefficient=152,000M-1cm-1;A280correctionfactoris0.19.
HaloTagisatrademarkofPromegaCorporation,andSNAP-tagisatrademarkofNewEnglandBiolabs,Inc.
SoldunderlicensefromtheHowardHughesMedicalInstitute,JaneliaResearchCampus
Super-resolutionimage(dSTORM)ofmuscletissuefromtheleftventricleofanadultpigheart.JF646(Cat.No.6148)wasusedtolabelcollagenVI,whichispresentonthecellplasmamembrane,andimagedwitha642nmexcitationlaser.Aclearerstructureisachievedincomparisonwiththewidefieldimage(topright).Scalebar2μm.
ImagekindlyprovidedbyProf.ChristianSoeller,UniversityofExeter;acquiredbyAlexClowsleyandAnnaMeletiou.
M.Wt | 593.71 |
Formula | C33H31N3O6Si |
Storage | Storeat-20°C |
CASNumber | 1811539-59-9 |
PubChemID | 124201858 |
InChIKey | ODBCGJUBBYOJOS-UHFFFAOYSA-N |
Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CCC7)=C2 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solvent | MaxConc.mg/mL | MaxConc.mM | |
---|---|---|---|
Solubility | |||
DMSO | 59.37 | 100 |
Thefollowingdataisbasedontheproductmolecularweight593.71.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
---|---|---|---|
1mM | 1.68mL | 8.42mL | 16.84mL |
5mM | 0.34mL | 1.68mL | 3.37mL |
10mM | 0.17mL | 0.84mL | 1.68mL |
50mM | 0.03mL | 0.17mL | 0.34mL |