Histonelysinedemethylase(KDM)inhibitor;blocksdemethylationofhistoneH3K27.Attenuateslipopolysaccharide(LPS)-inducedproinflammatorycytokineproductioninprimaryhumanmacrophages(IC50=9μMfortheinhibitionofTNFαrelease).Cellpermeable.EthylesterderivativeofGSKJ1(Cat.No.4593).
ThisprobeissuppliedinconjunctionwiththeStructuralGenomicsConsortium.ForfurthercharacterizationdetailsofGSKJ4,acellpermeablederivativeofGSKJ1,pleasevisittheGSKJ1probesummaryontheSGCwebsite.
Chemicalprobes.orgisaportalthatoffersindependentguidanceontheselectionand/orapplicationofsmallmoleculesforresearch.TheuseofGSKJ4isreviewedonthechemicalprobeswebsite.
GSKJ4isalsoofferedaspartoftheTocriscreenPlusandTocriscreenEpigeneticsToolbox.FindoutmoreaboutcompoundlibrariesavailablefromTocris.
M.Wt | 417.5 |
Formula | C24H27N5O2 |
Storage | StoreatRT |
Purity | ≥99%(HPLC) |
CASNumber | 1373423-53-0 |
PubChemID | 71729975 |
InChIKey | WBKCKEHGXNWYMO-UHFFFAOYSA-N |
Smiles | O=C(CCNC1=NC(C2=CC=CC=N2)=NC(N3CCC(C=CC=C4)=C4CC3)=C1)OCC |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solvent | MaxConc.mg/mL | MaxConc.mM | |
---|---|---|---|
Solubility | |||
DMSO | 41.75 | 100 | |
ethanol | 41.75 | 100 |
Thefollowingdataisbasedontheproductmolecularweight417.5.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
---|---|---|---|
1mM | 2.4mL | 11.98mL | 23.95mL |
5mM | 0.48mL | 2.4mL | 4.79mL |
10mM | 0.24mL | 1.2mL | 2.4mL |
50mM | 0.05mL | 0.24mL | 0.48mL |