Mimicofgreenfluorescentprotein(GFP)fluorophoreforimagingRNAinlivingcells.FluorescenceactivatedbybindingtoSpinach4orBroccoliaptamers.Exhibitspeakexcitationmaximaof482nmandpeakfluorescenceemissionof505nmwhenboundtoSpinach4,enablingimagingwithGFPfiltercubes.ExhibitshigherspecificfluorescenceandlowerbackgroundfluorescencewhenboundtoSpinach4comparedwithSpinach4-DFHBI.
M.Wt | 320.21 |
Formula | C13H9F5N2O2 |
Storage | Storeat-20°C |
Purity | ≥98%(HPLC) |
CASNumber | 1539318-36-9 |
PubChemID | 101889712 |
InChIKey | AWYCLBWNRONMQC-WMZJFQQLSA-N |
Smiles | O=C1N(CC(F)(F)F)C(C)=NC1=C/C2=CC(F)=C(O)C(F)=C2 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solvent | MaxConc.mg/mL | MaxConc.mM | |
---|---|---|---|
Solubility | |||
DMSO | 32.02 | 100 | |
ethanol | 16.01 | 50 |
Thefollowingdataisbasedontheproductmolecularweight320.21.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
---|---|---|---|
1mM | 3.12mL | 15.61mL | 31.23mL |
5mM | 0.62mL | 3.12mL | 6.25mL |
10mM | 0.31mL | 1.56mL | 3.12mL |
50mM | 0.06mL | 0.31mL | 0.62mL |