HighaffinityfluorescentlytaggedaptamerforCD133(extracellulardomaineepitope)(Kdvaluesare52.3and145.1nMinHep3BandHT29cells,respectively).EfficientlyinternalizeduponbindingtoCD133.Exhibitssuperiorpenetrationoftumorspherescomparedwithanantibodytothesametarget.
Foroptimalbindingaptamersrequirerefoldingintotheirtertiarystructurepriortouse.Pleaserefertotheaccompanyingrefoldingprotocol.
Soldunderlicensefromthepatentholder,DeakinUniversity.
M.Wt | 6847.32 |
Formula | C221H263F10N73O131P20 |
Storage | Storeat-20°C |
Aptamertype(DNA/RNA) | RNA |
NumberofBases | 19 |
SequenceModifications | 2-fluoropyrimidines,3'-inverteddeoxythymidinecap,5'-fluorescentDY647tag |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
PleaserefertothesesolubilityguidelinesforCD133-B19,5'-DY647(Cat.No.6104).
Thefollowingdataisbasedontheproductmolecularweight6847.32.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
---|---|---|---|
1mM | 0.15mL | 0.73mL | 1.46mL |
5mM | 0.03mL | 0.15mL | 0.29mL |
10mM | 0.01mL | 0.07mL | 0.15mL |
50mM | 0mL | 0.01mL | 0.03mL |