EnhancesCRISPR-Cas9-mediatedhomology-directedrepair(HDR)efficiencyinvitroupto19-fold.Inhibitsnonhomologousend-joining(NHEJ).
WehavediscoveredthatthecommerciallyavailableSCR7materialsuppliedbyothervendorsdoesnotmatchthechemicalstructurethatitisbeingsoldunder.ThroughdetailedchemicalanalysiscarriedoutbyourAnalyticalQualityControlexperts,webelievethatthecommerciallyavailableSCR7material,includingthatusedinrecentpublicationstoenhanceCRISPRefficiency,isinfactananalogofSCR7thatwehavenamed'SCR7pyrazine'.
ViewmoreinformationregardingthestructureofSCR7.
M.Wt | 332.38 |
Formula | C18H12N4OS |
Storage | Storeat+4°C |
Purity | ≥98%(HPLC) |
CASNumber | 14892-97-8 |
PubChemID | 10688007 |
InChIKey | GSRTWXVBHGOUBU-UHFFFAOYSA-N |
Smiles | O=C(C(N=C(C3=CC=CC=C3)C(C4=CC=CC=C4)=N2)=C2N1)NC1=S |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solvent | MaxConc.mg/mL | MaxConc.mM | |
---|---|---|---|
Solubility | |||
DMSO | 33.44 | 100 | |
ethanol | 6.69 | 20 |
Thefollowingdataisbasedontheproductmolecularweight332.38.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
---|---|---|---|
1mM | 3.01mL | 15.04mL | 30.09mL |
5mM | 0.6mL | 3.01mL | 6.02mL |
10mM | 0.3mL | 1.5mL | 3.01mL |
50mM | 0.06mL | 0.3mL | 0.6mL |
Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.