ReversIBLeinhibitorofproteintranslocationfromtheendoplasmicreticulum(ER)totheGolgiapparatus.BlocksbindingofADP-ribosylationfactortotheGolgiapparatusandinhibitsGDP-GTPexchange.Canbeusedtoinduceautophagyinmammaliancells.AlsoenhancesCRISPR-mediatedhomology-directedrepair(HDR)efficiency~2-foldwhenappliedat100nM,inhumaninducedpluripotentstemcells(iPSCs).
BrefeldinAisalsoofferedaspartoftheTocriscreenPlus.FindoutmoreaboutcompoundlibrariesavailablefromTocris.
M.Wt | 280.36 |
Formula | C16H24O4 |
Storage | Desiccateat-20°C |
Purity | ≥98%(HPLC) |
CASNumber | 20350-15-6 |
PubChemID | 6436187 |
InChIKey | KQNZDYYTLMIZCT-KFKPYADVSA-N |
Smiles | [H][C@]1(C)CCCC=CC2C[C@H](O)C[C@H]2[C@H](O)C=CC(=O)O1 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solvent | MaxConc.mg/mL | MaxConc.mM | |
---|---|---|---|
Solubility | |||
DMSO | 14.02 | 50 | |
ethanol | 2.8 | 10 |
Thefollowingdataisbasedontheproductmolecularweight280.36.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
---|---|---|---|
1mM | 3.57mL | 17.83mL | 35.67mL |
5mM | 0.71mL | 3.57mL | 7.13mL |
10mM | 0.36mL | 1.78mL | 3.57mL |
50mM | 0.07mL | 0.36mL | 0.71mL |
Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.